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Sample GSM5159490 Query DataSets for GSM5159490
Status Public on Mar 11, 2024
Title Mes3
Sample type RNA
 
Source name non-NE small cell lung cancer
Organism Mus musculus
Characteristics cell type: Mes cell line
genetic backgroud: RbL/L, Trp53L/L
Treatment protocol no treatment
Growth protocol RPMI1640+10%FBS
Extracted molecule total RNA
Extraction protocol Total RNAs from the Mes and Epi cell lines were extracted by TRIzol reagent (Life Technologies) according to the manufacturer’s instruction.
Label Cy3
Label protocol Total RNAs were amplified and labeled by Low Input Quick Amp Labeling Kit, One-Color (Agilent technologies, Santa Clara, CA, US), following the manufacturer’s instructions.
 
Hybridization protocol Each slide was hybridized with 1.65mg Cy3-labeled cRNA using Gene Expression Kit (Agilent technologies, Santa Clara, CA, US), according to the manufacturer’s instructions.
Scan protocol After 17 hours hybridization, slides were washed in staining dishes (Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit (Agilent technologies, Santa Clara, CA, US) following the manufacturer’s instructions. Slides were scanned by Agilent Microarray Scanner (Agilent technologies, Santa Clara, CA, US) with default settings, Dye channel: Green, Scan resolution = 5 mm, PMT 100%, 16 bits.
Data processing Data were extracted with Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US). Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US). GSEA was performed following the developer’s protocol (http://www.broad.mit.edu/gsea/).
 
Submission date Mar 11, 2021
Last update date Mar 11, 2024
Contact name yujuan jin
E-mail(s) jinyujuan1@163.com
Organization name Shanghai Institutes for Biological Sciences, CAS
Lab Hongbin Ji
Street address Yueyang Road 320#
City Shanghai
ZIP/Postal code 200031
Country China
 
Platform ID GPL7202
Series (1)
GSE168709 mRNA expression data of Mes vs. Epi cells in mouse non-NE SCLC

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 12.92487
DarkCorner 0.96738094
A_52_P616356 1.0069451
A_52_P580582 4.8157363
A_52_P403405 1.0175003
A_52_P819156 4.2541623
A_51_P331831 1.0257895
A_51_P430630 1.0332842
A_52_P502357 1.0373552
A_52_P299964 5.8581305
A_51_P356389 4.43016
A_52_P684402 8.578657
A_51_P414208 1.0481552
A_51_P280918 9.230843
A_52_P613688 4.67513
A_52_P258194 1.0552957
A_52_P229271 7.0766687
A_52_P214630 7.2444615
A_52_P579519 6.6804094
A_52_P979997 1.0622659

Total number of rows: 41267

Table truncated, full table size 912 Kbytes.




Supplementary file Size Download File type/resource
GSM5159490_Mes3_251486836780_S01_GE1_107_Sep09_1_3.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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