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| Status |
Public on Sep 25, 2021 |
| Title |
Single-cell RNA-Seq of premature Rhesus macaques lungs with intra-uterine inflammation and therapuetic blockade |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Intrauterine inflammation/infection (IUI), which is present in up to 40% of premature births, leads to elevated levels of pro-inflammatory mediators and microbial products within the amniotic fluid, which come in close contact to fetal mucosae. Yet, knowledge on the fetal mucosal responses to IUI exposure remains limited. To address these questions, we used a non-human primate model of IUI, in which pregnant Rhesus macaques received intra-amniotic (IA) LPS, compared with saline IA. We found that IA LPS exposure induced a robust and rapid inflammation of the fetal lung.
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| Overall design |
Single-cell RNA-Seq was performed in the premature Rhesus macaques lung in the presence and absence of LPS. Therapuetic intervention was tested in LPS animals with IL-1R, TNF-alpha or combination blockade.
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| Contributor(s) |
Jackson CM, Mukherjee S, Senthamaraikannan P, Presicce P, Kallapur SG, Miller LA, Lewkowich IP, Demmert M, Salomonis N, Jobe AH, Zacharias WJ, Chougnet CA |
| Citation(s) |
35353543 |
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| Submission date |
Mar 22, 2021 |
| Last update date |
Jul 11, 2022 |
| Contact name |
Nathan Salomonis |
| E-mail(s) |
nathan.salomonis@cchmc.org
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| Organization name |
Cincinnati Children's Hospital
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| Department |
Biomedical Informatics
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| Lab |
Nathan Salomonis
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| Street address |
3333 Burnet Avenue
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| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL27943 |
Illumina NovaSeq 6000 (Macaca mulatta) |
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| Samples (13)
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| GSM5201847 |
fetal lungs, LPS-2 |
| GSM5201848 |
fetal lungs, TNF-alpha-blockade-1 |
| GSM5201849 |
fetal lungs, TNF-alpha-blockade-2 |
| GSM5201850 |
fetal lungs, TNF-alpha-blockade-3 |
| GSM5201851 |
fetal lungs, ILRA-blockade-1 |
| GSM5201852 |
fetal lungs, ILRA-blockade-2 |
| GSM5201853 |
fetal lungs, ILRA-blockade-3 |
| GSM5208108 |
fetal lungs, Combo-blockade-1 |
| GSM5208109 |
fetal lungs, Combo-blockade-2 |
| GSM5208110 |
fetal lungs, Combo-blockade-3 |
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| Relations |
| BioProject |
PRJNA716367 |
| SRA |
SRP311767 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE169390_RAW.tar |
285.5 Mb |
(http)(custom) |
TAR (of H5) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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