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| Status |
Public on Mar 04, 2010 |
| Title |
MRC1-Dependent Scaling of the Budding Yeast DNA Replication Timing Program |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Fourteen yeast mutants with an extended S-phase were identified by a novel genome-wide screen. These mutants are associated with the DNA replication machinery, cell-cycle control and dNTP synthesis. We determined the genome-wide DNA replication timing profile of all these mutants as well as wild type, by FACS-sorting G1- and S-phase cells and co-hybridizing their DNA to Agilent genomic tiling arrays, in four repeats each. We find that MRC1 is required for scaling of the DNA replication timing program upon replication perturbation.
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| Overall design |
Four repeats for each strain, S vs G1 phase-cell DNA with different Cy dyes for each
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| Contributor(s) |
Koren A |
| Citation(s) |
20219942 |
| Submission date |
Jul 15, 2009 |
| Last update date |
Feb 15, 2018 |
| Contact name |
Amnon Koren |
| E-mail(s) |
koren@genetics.med.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Department |
Genetics
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL4131 |
Agilent-014810 Yeast Whole Genome ChIP-on-Chip Microarray 4x44K (G4493A) |
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| Samples (58)
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| Relations |
| BioProject |
PRJNA119717 |
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