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| Status |
Public on Apr 06, 2021 |
| Title |
Transcriptome analysis reveals the contribution of glia -derived cues for maintenance of microglia identity in rhesus macaques |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We analyzed the transcriptomes of different primary microglia cultures from adult rhesus macaques, and compared them with those of ex vivo microglia
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| Overall design |
Primary microglia cultured with M-CSF, IL-34, M-CSF + TGFb, IL-34 + TGFb (n=4). Primary microglia cultured with serum + M-CSF, serum (4days) + different serum-free wash out regimes (4, 11 and 18 days), and serum-free medium (22 days) (n=4). Primary microglia cultured with serum + M-CSF, with serum for 4 days and subsequently cultured in serum-free medium for 11 days, and microglia cultured in spheres together with oligodendrocytes and radial glia (n=4). Freshly isolated microglia (ex vivo) (n=4)
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| Contributor(s) |
Timmerman R, Bajramovic JJ |
| Citation missing |
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| |
| Submission date |
Apr 05, 2021 |
| Last update date |
Apr 15, 2021 |
| Contact name |
Raissa Timmerman |
| E-mail(s) |
raissatimmerman@gmail.com
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| Organization name |
Biomedical Primate Research Centre
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| Department |
Alternatives
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| Street address |
Lange Kleiweg 161
|
| City |
Rijswijk |
| ZIP/Postal code |
2288 GJ |
| Country |
Netherlands |
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| Platforms (2) |
| GPL21120 |
Illumina NextSeq 500 (Macaca mulatta) |
| GPL27943 |
Illumina NovaSeq 6000 (Macaca mulatta) |
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| Samples (52)
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| Relations |
| BioProject |
PRJNA719784 |
| SRA |
SRP313472 |
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