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| Status |
Public on Nov 15, 2021 |
| Title |
Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species |
| Organisms |
Sus scrofa; Bos taurus; Ovis aries |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Despite four decades of effort, robust propagation of pluripotent stem cells from livestock animals remains challenging. The requirements for self-renewal are unclear and the relationship of cultured stem cells to pluripotent cells resident in the embryo uncertain. Here we avoided feeder cells or serum factors to provide a defined culture microenvironment. We show that the combination of Activin A, Fibroblast growth factor, and Wnt inhibitor XAV939 (AFX), supports establishment and continuous expansion of pluripotent stem cell lines from porcine, ovine and bovine embryos. Germ layer differentiation was evident in teratomas and readily induced in vitro. Global transcriptome analyses highlighted commonality in transcription factor expression across the three species, while global comparison with porcine embryo stages showed proximity to bilaminar disc epiblast. Clonal genetic manipulation and gene targeting were exemplified in porcine stem cells. We further demonstrated that genetically modified AFX stem cells gave rise to cloned porcine foetuses by nuclear transfer. In summary, for major livestock mammals pluripotent stem cells related to the formative embryonic disc are reliably established using a common and defined signaling environment.
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| Overall design |
RNA-seq analysis of EDSCs established from pig, sheep and cow embryos
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| |
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| Contributor(s) |
Kinoshita M, Spindlow D, Bornelöv S, Stirparo GG, Smith A |
| Citation(s) |
34874452 |
| Submission date |
Apr 20, 2021 |
| Last update date |
Jan 11, 2022 |
| Contact name |
Daniel Spindlow |
| Organization name |
University of Exeter
|
| Department |
Living Systems Institute
|
| Lab |
Smith Lab
|
| Street address |
Stocker Road
|
| City |
Exeter |
| State/province |
Devon |
| ZIP/Postal code |
EX4 4QD |
| Country |
United Kingdom |
| |
|
| Platforms (3) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
| GPL26351 |
Illumina NovaSeq 6000 (Sus scrofa) |
| GPL27721 |
Illumina NovaSeq 6000 (Ovis aries) |
|
| Samples (33)
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|
| Relations |
| BioProject |
PRJNA723284 |
| SRA |
SRP315554 |
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