|
| Status |
Public on Nov 15, 2021 |
| Title |
bovine Line 6 p17 |
| Sample type |
SRA |
| |
|
| Source name |
b6
|
| Organism |
Bos taurus |
| Characteristics |
cell type: EDSCs
passage: 17
|
| Treatment protocol |
N/A
|
| Growth protocol |
EDSCs were cultured in AFX medium on Laminin/Fibronectin coated dish.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed in Trizol and total RNAs are prepared by PureLink RNA Mini Kit
Libraries were prepared by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Cow_EDSC_raw_counts.txt
bovine Line 6 p17
|
| Data processing |
Trim-Galore!
STAR
FeatureCounts
Supplementary_files_format_and_content: raw counts text file
|
| |
|
| Submission date |
Apr 20, 2021 |
| Last update date |
Nov 15, 2021 |
| Contact name |
Daniel Spindlow |
| Organization name |
University of Exeter
|
| Department |
Living Systems Institute
|
| Lab |
Smith Lab
|
| Street address |
Stocker Road
|
| City |
Exeter |
| State/province |
Devon |
| ZIP/Postal code |
EX4 4QD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL26012 |
| Series (1) |
| GSE172420 |
Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species |
|
| Relations |
| BioSample |
SAMN18810518 |
| SRA |
SRX10644303 |
