|
| Status |
Public on Aug 30, 2021 |
| Title |
Identify molecules regulated by glucosylceramide synthase in mouse osteoblasts. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify molecules regulated by glucosylceramide synthase in osteoblasts, we conducted microarray analysis using samles treated with the inhibitors of glucosylceramide synthase.
|
| |
|
| Overall design |
Four groups for MC3T3-E1 cells were Control, Miglustat, D-PDMP, and D-PPMP. The concentrations of Miglustat, D-PDMP, and D-PPMP were 50 microM, 10 microM, and 1.0 microM, respectively. Four days after treatment of their inhibitors of GCS, the cells were plated at 2.5 x 100000 cells into 60 mm dishes, and cultured for 24 h. The cells were harvested for microarray analysis.
|
| |
|
| Contributor(s) |
Hamamura K |
| Citation(s) |
34697142 |
| Submission date |
May 12, 2021 |
| Last update date |
Nov 29, 2021 |
| Contact name |
Kazunori Hamamura |
| E-mail(s) |
hamak@dpc.agu.ac.jp
|
| Organization name |
Aichi Gakuin University, School of Dentistry
|
| Department |
Pharmacology
|
| Street address |
1-100 Kusumoto-cho, Chikusa-ku
|
| City |
Nagoya |
| ZIP/Postal code |
464-8650 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA729440 |
Less...
More...