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| Status |
Public on Jul 04, 2023 |
| Title |
Methylome dynamic upon proteasome inhibition by the pseudomonas virulence factor Syringolin A |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
DNA methylation is an important epigenetic mark required for proper gene expression and silencing of transposable elements. DNA methylation patterns can be modified by environmental factors such as pathogen infection, where modification of DNA methylation can be associated with plant resistance. To counter the plant defense pathways, pathogens produce effectors molecule, several of which act as proteasome inhibitors. Here we investigated the effect of proteasome inhibition by the bacterial virulence factor Syringolin A on genome-wide DNA methylation. We show that Syringolin A treatment results in an increase of DNA methylation at centromeric and pericentromeric regions of Arabidopsis chromosomes. We identify several CHH DMRs that are enriched in the proximity of transcriptional start sites. Syringolin A treatment does not result in significant changes in small RNA composition. However, significant changes in genome transcriptional activity can be observed, including a strong upregulation of resistance genes that are located on chromosomal arms. We hypothesize that DNA methylation changes could be linked to the upregulation of some atypical members of the de novo DNA methylation pathway: AGO3, AGO9 and DRM1. Our data suggests that modification of genome-wide DNA methylation resulting from an inhibition of the proteasome by bacterial effectors could be part of a epi-genomic arms race against pathogens.
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| Overall design |
GWBS, mRNA and sRNA sequencing of wildtype Arabidopsis plantlets treated or not with SyringolinA
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| Contributor(s) |
Jullien PE, Grob S |
| Citation missing |
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| Submission date |
May 12, 2021 |
| Last update date |
Jul 05, 2023 |
| Contact name |
Stefan Grob |
| E-mail(s) |
sgrob@botinst.uzh.ch
|
| Organization name |
University of Zurich
|
| Department |
Institute of Plant Biology
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| Lab |
Grossniklaus Lab
|
| Street address |
Zollikerstrasse 107
|
| City |
Zurich |
| ZIP/Postal code |
8008 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL21785 |
Illumina HiSeq 4000 (Arabidopsis thaliana) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA729499 |
| SRA |
SRP319525 |
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