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| Status |
Public on Mar 01, 2010 |
| Title |
KDM7 is a dual demethylase for histone H3 lysines 9 and 27 and functions in brain development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The roles of histone demethylase KDM7 in gene expression were analyzed by gene expression profiling experiments with the mouse neuroblastoma cell line Neuro2A.
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| Overall design |
In order to examine the effect of KDM7 in gene expression, we generated stable KDM7 knockdown cell lines in the mouse neuroblastoma cell line Neuro2A. Total RNAs were extracted from 5 cell lines and analyzed for gene expression profiles using the Affymetrix platform.
Cell lines:
Parental cells: Neuro2A (N2A)
empty vector: Neuro2A transfected with empty vector
EGFP KD: Neuro2A transfected with vector for EGFP knock down
KDM7 KD1: Neuro2A transfected with vector 1 for KDM7 knock down
KDM7 KD2: Neuro2A transfected with vector 2 for KDM7 knock down
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| Contributor(s) |
Tsukada Y |
| Citation(s) |
20194436 |
| |
| Submission date |
Aug 04, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Yuichi Tsukada |
| E-mail(s) |
ytsukada@bioreg.kyushu-u.ac.jp
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| Phone |
+81-092-642-6820
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| Fax |
+81-092-642-6819
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| Organization name |
Kyushu university
|
| Department |
Medical Institute of Bioregulation
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| Street address |
3-1-1 Maidashi, Higashi-ku
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| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA118929 |
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