|
| Status |
Public on Mar 01, 2010 |
| Title |
N2A EGFP KD |
| Sample type |
RNA |
| |
|
| Source name |
Neuro2A transfected with vector for EGFP knock down
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Neuro2A (N2A)
cell type: neuroblastoma
|
| Growth protocol |
Srandard culture condition for Neuro2A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells and purified with the use of Qiashredder spin-columns (Qiagen) and an RNeasy Plus Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS3000 scanner.
|
| Description |
neuroblastoma cell line Neuro2A transfected with vector for EGFP knock down
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
|
| |
|
| Submission date |
Aug 04, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yuichi Tsukada |
| E-mail(s) |
ytsukada@bioreg.kyushu-u.ac.jp
|
| Phone |
+81-092-642-6820
|
| Fax |
+81-092-642-6819
|
| Organization name |
Kyushu university
|
| Department |
Medical Institute of Bioregulation
|
| Street address |
3-1-1 Maidashi, Higashi-ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE17494 |
KDM7 is a dual demethylase for histone H3 lysines 9 and 27 and functions in brain development |
|
| Relations |
| Reanalyzed by |
GSE119085 |
