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| Status |
Public on May 20, 2024 |
| Title |
Cell-in-Cell Structure Mediates In-Cell Killing Suppressed by CD44 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Penetration of immune cells into tumor cells was believed to be immune-suppressive via cell-in-cell (CIC) mediated death of the internalized immune cells. We unexpectedly found that CIC formation largely led to the death of the host tumor cells, but not the internalized immune cells, manifesting typical features of death executed by NK cells, which we called “in-cell killing” that demonstrates efficacy superior to the canonical way of “kiss killing” from outside. By expression profiling of isogenic cells, CD44 on tumor cells was identified as a negative regulator of “in-cell killing” via inhibiting CIC formation. CD44 functions to antagonize NK cell internalization by reducing N-cadherin-mediated intercellular adhesion and enhancing Rho GTPase-regulated cellular stiffness as well. Remarkably, antibody-mediated blockade of CD44 signaling potentiated the suppressive effects of NK cells on tumor growth associated with increased heterotypic CIC formation. Together, we identified CIC-mediated “in-cell killing” as a promising strategy for cancer immunotherapy.
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| Overall design |
Examination of genes associated to cell-in-cell formation in liver tumor cells.
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| Contributor(s) |
Sun Q, Fan J, Liang J, Su Y |
| Citation missing |
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| |
| Submission date |
May 30, 2021 |
| Last update date |
May 20, 2024 |
| Contact name |
Qiang Sun |
| E-mail(s) |
sunq@bmi.ac.cn
|
| Phone |
18201356998
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| Organization name |
Institute of Biotechnology
|
| Department |
Laboratory of Cell Engineering
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| Lab |
Laboratory of Cell Engineering
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| Street address |
20 Dongda Street
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100071 |
| Country |
China |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA733823 |
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