|
| Status |
Public on May 20, 2024 |
| Title |
I7S |
| Sample type |
RNA |
| |
|
| Source name |
Liver tumor cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PLC/PRF/5
treatment: Untreated
|
| Treatment protocol |
None
|
| Growth protocol |
Cell line PLC/PRF/5 and its derivatives were routinely maintained in Dulbecco’s modified Eagle’s medium (MACGENE Technology Ltd., Beijing, China) supplemented with 10% fetal bovine serum (Kangyuan Biology, China), and 100 units/mL penicillin plus 100 µg/mL streptomycin (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
|
| |
|
| Hybridization protocol |
DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was performed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
|
| Data processing |
Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and GeneSpring software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
May 30, 2021 |
| Last update date |
May 20, 2024 |
| Contact name |
Qiang Sun |
| E-mail(s) |
sunq@bmi.ac.cn
|
| Phone |
18201356998
|
| Organization name |
Institute of Biotechnology
|
| Department |
Laboratory of Cell Engineering
|
| Lab |
Laboratory of Cell Engineering
|
| Street address |
20 Dongda Street
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100071 |
| Country |
China |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE175819 |
Cell-in-Cell Structure Mediates In-Cell Killing Suppressed by CD44 |
|
