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| Status |
Public on Sep 15, 2021 |
| Title |
Transcriptional profiling of canine acanthomatous ameloblastoma |
| Organism |
Canis lupus familiaris |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Ameloblastomas are odontogenic tumors that are rare in people but have a relatively high prevalence in dogs. Because canine acanthomatous ameloblastomas (CAA) have clinicopathologic and molecular features in common with human ameloblastomas (AM), spontaneous CAA can serve as a useful translational model of disease. However, the molecular basis of CAA and how it compares to AM are incompletely understood. In this study, we compared the global genomic expression profile of CAA with AM and evaluated their dental origin by using a bulk RNA-seq approach. For these studies, healthy gingiva (HGIN) and canine oral squamous cell carcinoma (COSCC) served as controls. We found that aberrant RAS signaling, and activation of the epithelial-to-mesenchymal transition cellular program are involved in the pathogenesis of CAA, and that CAA is enriched with genes known to be upregulated in AM including those expressed during the early stages of tooth development, suggesting a high level of molecular homology. These results support the model that domestic dogs with spontaneous CAA have potential for pre-clinical assessment of targeted therapeutic modalities against AM.
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| Overall design |
A total of 25 canine tissue samples, representing 12 CAA, 8 COSCC and 5 HGIN samples, were profiled with RNA-seq. The 12 CAA samples are represented by SRA BioProject accession #PRJNA533473.
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| Contributor(s) |
Peralta S, Grenier JK |
| Citation(s) |
34493785 |
| BioProject |
PRJNA533473 |
| |
| Submission date |
Jun 01, 2021 |
| Last update date |
Sep 15, 2021 |
| Contact name |
Jennifer K Grenier |
| Organization name |
Cornell University
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| Department |
Biomedical Sciences
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| Lab |
Biotechnology Building rm 333
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| Street address |
526 Campus Rd
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| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (1) |
| GPL21400 |
Illumina NextSeq 500 (Canis lupus familiaris) |
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| Samples (25)
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