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| Status |
Public on Feb 15, 2024 |
| Title |
Linker histone H1 drives heterochromatin condensation via phase separation in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Other
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| Summary |
We studied how H1 regulates higher-order chromatin architecture by performing Hi-C. In addition, we tested the effects of H1 domains on nucleosome organisation and DNA methylation by performing MNase-seq and Bisulfite-seq.
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| Overall design |
Hi-C was performed with h1 mutant seedlings to examine chromatin interactions in the mutant. To test the functions of the C-terminal intrinsically disordered region (IDR) of H1, we generated H1.1G18IDR which contains the IDR and 18 amino acids of the globular domain nearest the IDR in h1 background. As a control, H1.2 with IDR deleted (H1.2ΔIDR) in h1 background was also generated. These transgenic plants as well as WT and h1 mutant were subject to MNase-seq and Bisulfite-seq to analyse nucleosome repeat length and DNA methylation.
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| Contributor(s) |
He S, Yu Y, Wang L, Zhang J, Bai Z, Li G, Li P, Feng X |
| Citation(s) |
38309957 |
| |
| Submission date |
Jun 10, 2021 |
| Last update date |
Feb 16, 2024 |
| Contact name |
Xiaoqi Feng |
| E-mail(s) |
xiaoqi.feng@jic.ac.uk
|
| Organization name |
John Innes Centre
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| Department |
Cell and Developmental Biology
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| Lab |
Xiaoqi Feng
|
| Street address |
Norwich Research Park
|
| City |
Norwich |
| State/province |
Norfolk |
| ZIP/Postal code |
NR4 7UH |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
| GPL23157 |
HiSeq X Ten (Arabidopsis thaliana) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA736615 |
| SRA |
SRP323534 |
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