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Series GSE17701 Query DataSets for GSE17701
Status Public on Sep 15, 2015
Title Genome analysis of C. jejuni and C. coli isolated from different regions of the same pig
Platform organisms Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; Campylobacter jejuni RM1221; Campylobacter coli RM2228
Sample organisms Campylobacter coli; Campylobacter jejuni; Campylobacter jejuni RM1221
Experiment type Genome variation profiling by array
Summary C. coli is the predominant Campylobacter strain that is found in pigs while C. jejuni if present will be 10- 100 folds less. Natural transformation can occur if there is a coexistence of both the strains in the intestine of pigs. Genome analyses were performed on a C. jejuni strain U101 isolated from the upper intestine and two C. coli strains, C101 and L101 isolated from caecum and lower intestine.
Overall design Five independent DNA preps of C. jejuni RM1221 were labelled with Cy 5 independently and they were mixed well which was used as the control. C101, U101 and L101 were labelled with Cy 3 independently and equal concentration of the control and sample DNA were used for hybridisation. Three biological replicates were done for each slide.
The supplementary file (linked at the foot of this record) represents a summary of the C101, L101, and U101 replicate data (3 replicates/strain) that have been averaged.
Contributor(s) John A, Timms A, Connerton P, Connerton I
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Submission date Aug 18, 2009
Last update date Sep 15, 2015
Contact name Amy John
Organization name University of Nottingham
Street address Loughborough
City Leicestershire
ZIP/Postal code LE12 5RD
Country United Kingdom
Platforms (1)
GPL9022 UON_Campylobacter oligo array v1
Samples (9)
GSM441396 1221-U101 replicate 1
GSM441397 1221-C101 replicate 1
GSM441398 1221-L101 replicate 1
BioProject PRJNA118409

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17701_RAW.tar 15.1 Mb (http)(custom) TAR (of GPR)
GSE17701_summary_C1_L1_U1.txt.gz 365.0 Kb (ftp)(http) TXT
Processed data included within Sample table

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