|
| Status |
Public on Sep 15, 2015 |
| Title |
Genome analysis of C. jejuni and C. coli isolated from different regions of the same pig |
| Platform organisms |
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; Campylobacter jejuni RM1221; Campylobacter coli RM2228 |
| Sample organisms |
Campylobacter coli; Campylobacter jejuni; Campylobacter jejuni RM1221 |
| Experiment type |
Genome variation profiling by array
|
| Summary |
C. coli is the predominant Campylobacter strain that is found in pigs while C. jejuni if present will be 10- 100 folds less. Natural transformation can occur if there is a coexistence of both the strains in the intestine of pigs. Genome analyses were performed on a C. jejuni strain U101 isolated from the upper intestine and two C. coli strains, C101 and L101 isolated from caecum and lower intestine.
|
| |
|
| Overall design |
Five independent DNA preps of C. jejuni RM1221 were labelled with Cy 5 independently and they were mixed well which was used as the control. C101, U101 and L101 were labelled with Cy 3 independently and equal concentration of the control and sample DNA were used for hybridisation. Three biological replicates were done for each slide.
The supplementary file (linked at the foot of this record) represents a summary of the C101, L101, and U101 replicate data (3 replicates/strain) that have been averaged.
|
| |
|
| Contributor(s) |
John A, Timms A, Connerton P, Connerton I |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 18, 2009 |
| Last update date |
Sep 15, 2015 |
| Contact name |
Amy John |
| E-mail(s) |
asj918@gmail.com
|
| Organization name |
University of Nottingham
|
| Street address |
Loughborough
|
| City |
Leicestershire |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL9022 |
UON_Campylobacter oligo array v1 |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA118409 |
Less...
More...