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| Status |
Public on Apr 01, 2010 |
| Title |
Biological variation between zebrafish development series |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptome analysis during embryogenesis usually requires pooling of embryos to obtain sufficient RNA. Hence, the measured levels of gene-expression represent the average mRNA levels of pooled samples and the biological variation among individuals is lost for inclusion into statistical models used to analyze transcriptome data. This can irreversibly reduce the robustness, resolution and expressiveness of the experiment. Therefore, we developed a robust method to isolate abundant high-quality RNA from individual embryos to perform single embryo transcriptome analyses using the zebrafish as a model.
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| Overall design |
RNA from 8 individual embryos (16-cell, 128-cell, sphere, dome, germ ring, shield, 75%-epiboly & 8-somite stage) was isolated and hybridized against a common reference (pool of these 8 samples).
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| Contributor(s) |
de Jong M, Rauwerda H, Bruning O, Verkooijen J, Spaink H, Breit T |
| Citation(s) |
20233395 |
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| Submission date |
Aug 20, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Mark de Jong |
| Organization name |
University of Amsterdam
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| Street address |
Kruislaan 318
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| City |
Amsterdam |
| ZIP/Postal code |
1098 SM |
| Country |
Netherlands |
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| Platforms (1) |
| GPL9060 |
Danio rerio dedicated 15k array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA118361 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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