|
| Status |
Public on Apr 01, 2010 |
| Title |
Shield |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Shield
|
| Organism |
Danio rerio |
| Characteristics |
strain: AB
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiazol & RNeasy (Qiagen)
Amino-Allyl MessageAmp II (Ambion)
|
| Label |
Cy3
|
| Label protocol |
Indirectly via Amino-Allyl-dUTP
|
| |
|
| Channel 2 |
| Source name |
Common reference pooled samples
|
| Organism |
Danio rerio |
| Characteristics |
strain: AB
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiazol & RNeasy (Qiagen)
Amino-Allyl MessageAmp II (Ambion)
|
| Label |
Cy5
|
| Label protocol |
Indirectly via Amino-Allyl-dUTP
|
| |
|
| |
| Hybridization protocol |
See Agilent Two-Color Microarray Based Gene Expression Analysis (v5.5)
|
| Scan protocol |
See Agilent Two-Color Microarray Based Gene Expression Analysis (v5.5)
Agilent G2565BA scanner
Scans performed at 5 um and XDR
|
| Description |
See Agilent Two-Color Microarray Based Gene Expression Analysis (v5.5)
|
| Data processing |
Median signals (gMedianSignal and rMedianSignal) were log2 transformed. Ratios were obtained by subtraction;
log2(gMedianSignal)-log2(rMedianSignal).
|
| |
|
| Submission date |
Aug 20, 2009 |
| Last update date |
Jan 23, 2010 |
| Contact name |
Mark de Jong |
| Organization name |
University of Amsterdam
|
| Street address |
Kruislaan 318
|
| City |
Amsterdam |
| ZIP/Postal code |
1098 SM |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL9060 |
| Series (1) |
| GSE17736 |
Biological variation between zebrafish development series |
|
