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| Status |
Public on Oct 30, 2009 |
| Title |
Gene expression analysis of zebrafish embryos depleted of arrb1 and/or arrb2 at 12 hpf |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
To comprehensively understand the genes affected by beta-arrestin1 depletion in zebrafish fish embryos at 12 hpf, microarray analysis was performed. As controls, zebrafish embryos depleted of beta-arrestin2 and double depletion of beta-arrestin1 and beta-arrestin2 were also analyzed.
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| Overall design |
Briefly, 50 AB/WT zebrafish embryos injected with 4ng control MO, 4ng arrb1 MO, 4ng arrb2 MO and 4ng arrb1+ 4ng arrb2 MO were lyzed in Trizol reagent at 12 hpf. Total RNAs were extracted, and subsequent operation was performed according to Agilent "One-Color Microarray-Based Gene Expression Analysis" protocol, version 5.5.
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| Contributor(s) |
Yue R, Liu X, Zhao C, Kang J, Pei G |
| Citation(s) |
19879840 |
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| Submission date |
Aug 24, 2009 |
| Last update date |
Nov 16, 2012 |
| Contact name |
Rui Yue |
| E-mail(s) |
ryue@sibs.ac.cn
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| Organization name |
Shanghai Institute of Biochemistry and Cell Biology
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| Street address |
320 Yueyang Road
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL7302 |
Agilent-015064 D. rerio (Zebrafish) Oligo Microarray 4x44K G2519F (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA118297 |
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