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| Status |
Public on Nov 24, 2021 |
| Title |
Gene analysis of tumor cells from mice with the Treg-specific deletion of Blimp1 compared to WT mice (NanoString Cancer) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We observed that mice with the Treg-specific deletion of Blimp1 had delayed and smaller tumor growth associated with the activation of tumor-infiltrating immune effector cells.
To determine what extent disruptions of Treg suppressive activity by a specific deletion of Blimp1 could impact on the tumor, we assessed the gene expression of sorted tumor cells by NanoString analysis.
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| Overall design |
Prdm1fl/flFoxP3YFP-Cre (KO) and FoxP3YFP-Cre (WT) mice were subcutaneously inoculated with B16.OVA at day 0, and intraperitoneally immunized with NP-OVA in CFA at day 0 and NP-OVA in IFA at day 7. When the tumor reached ≤ 1.5 cm3, CD45-live tumor cells were FACS-sorted from the tumors of these mice. RNA was prepared and analyzed using NanoString PanCancer Pathways Panel.
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| Contributor(s) |
Leavenworth JW |
| Citation(s) |
34798898 |
| |
| Submission date |
Jun 14, 2021 |
| Last update date |
Nov 24, 2021 |
| Contact name |
Jianmei Wu Leavenworth |
| E-mail(s) |
jleavenworth@uabmc.edu
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| Organization name |
University of Alabama at Birmingham
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| Department |
Neurosurgery, Microbiology
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| Lab |
Leavenworth Lab, CHB130
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| Street address |
1600 6th Ave South
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| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35233 |
| Country |
USA |
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| Platforms (1) |
| GPL28581 |
nCounter® PanCancer Mouse Pathways Panel |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE178135 |
Treg cells and Treg-specific deletion of Blimp1 |
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| Relations |
| BioProject |
PRJNA737441 |
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