GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE17815 Query DataSets for GSE17815
Status Public on Feb 09, 2010
Title Uridylation of mature miRNAs and siRNAs by the MUT68 nucleotidyltransferase promotes their degradation in Chlamydomonas
Organism Chlamydomonas reinhardtii
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Regulation of gene expression by small RNAs (∼20–30 nucleotides in length) plays an essential role in developmental pathways and defense responses against genomic parasites in eukaryotes. MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) commonly direct the inactivation of cognate sequences through a variety of mechanisms, including RNA degradation, translation inhibition, and transcriptional repression. Recent studies have provided considerable insight into the biogenesis and the mode of action of miRNAs and siRNAs. However, relatively little is known about mechanisms of quality control and small RNA decay in RNA interference (RNAi) pathways. Here we show that deletion of MUT68, encoding a terminal nucleotidyltransferase in the alga Chlamydomonas reinhardtii, results in elevated miRNA and siRNA levels. We found that MUT68 plays a role in the untemplated uridylation of the 3′ ends of small RNAs in vivo and stimulates their degradation by the RRP6 exosome subunit in vitro. Moreover, RRP6 depletion also leads to accumulation of small RNAs in vivo. We propose that MUT68 and RRP6 cooperate in the degradation of mature miRNAs and siRNAs, as a quality control mechanism to eliminate dysfunctional or damaged small RNA molecules.

Keywords: small RNA
Overall design Four small RNA libraries were created: two from Mut68 and two from the Maa7-IR44 control using unique barcodes at the 3' end of the 5' adapter. Libraries were pooled and sequenced.
Contributor(s) Ibrahim F, Rymarquis LA, Kim E, Becker J, Balassa E, Green PJ, Cerutti H
Citation(s) 20142471
Submission date Aug 26, 2009
Last update date Jun 11, 2013
Contact name Linda Rymarquis
Organization name University of Delaware
Street address 15 Innovation way rm 241
City Newark
State/province DE
ZIP/Postal code 19711
Country USA
Platforms (1)
GPL9100 Illumina Genome Analyzer II (Chlamydomonas reinhardtii)
Samples (4)
GSM444441 Mut-68_Chlamydomonas_rep1
GSM444442 Mut-68_Chlamydomonas_rep2
GSM444443 Maa7-IR44_Chlamydomonas_rep1
BioProject PRJNA120125

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap