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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 25, 2012 |
| Title |
Basal expression in daDREAM transgenic mouse hippocampus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Changes in nuclear Ca2+ homeostasis activate specific gene expression programs and are central to the acquisition and the plastic storage of memories. DREAM /KChIP proteins form heterotetramers that bind DNA and repress transcription in a Ca2+-dependent manner. Single ablation of one member of the DREAM/KChIP family may result in a mild or the absence of phenotype due to partial gene compensation. To study the function of DREAM/KChIP proteins in the brain, we used transgenic mice expressing a Ca2+-insensitive/CREB-independent dominant active mutant DREAM (daDREAM). We show that daDREAM controls the expression of several activity-dependent transcription factors including Npas4, Nr4a1, Mef2C, JunB and c-Fos, as well as the chromatin modifying enzyme Mbd4 and proteins related to actin polymerization like Arc and gelsolin. Thus, directly or through these targets, expression of daDREAM in the forebrain resulted in a complex phenotype characterized by i) impaired learning and memory, ii) loss of recurrent inhibition and enhanced LTP in the dentate gyrus without affecting Kv4-mediated potassium currents, and iii) modified spine density in DG granule neurons. Our results propose DREAM as a master-switch transcription factor regulating several activity-dependent gene expression programs to control synaptic plasticity, learning and memory.
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| Overall design |
We used Affymetrix microarrays (GeneChip Mouse Genome 430 2.0) to compare global gene expression in wild type (WT) versus transgenic hippocampi (L26 mice). For ech type of sample three hybridizations were carried-out (independent biological replicates).
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| Contributor(s) |
Mellström B, Knafo S, Sahún I, Ruiz-Nuño A, Murtra P, Oliveros JC, Higueras A, Li X, Gomez-Villafuerte R, Kastanauskaite A, Savignac M, Errington ML, Maldonado R, Zhuo M, Jefferys JG, DeFelipe J, Dierssen M, Bliss TV, Naranjo JR |
| Citation(s) |
24366545, 26928278 |
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| Submission date |
Aug 27, 2009 |
| Last update date |
Oct 08, 2019 |
| Contact name |
Juan Carlos Oliveros |
| Organization name |
CNB, CSIC
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| Street address |
Darwin 3
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| City |
Cantoblanco |
| State/province |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA117901 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17844_RAW.tar |
25.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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