|
| Status |
Public on Apr 25, 2012 |
| Title |
Hippocampus wild type rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampus, wild type
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Wild Type
tissue: Hippocampus
|
| Growth protocol |
Experiments were performed in adult male mice from two daDREAM transgenic lines, L1 and L26, homozygous for the transgene, and wild type littermates. The genetic background in all cases was C57B6xCBA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using TRIzol (Invitrogen) and the RNeasy Mini Kit (Qiagen). RNA was quantified and the RNA quality was assessed with a 2100 Bioanalyzer from Agilent Technologies
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 4 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 µg of biotinylated cRNA were hybridized to Affymetrix chips (GeneChip Mouse Genome 430 2.0 Array). Each sample was added to a hybridization solution containing 100mM 2-(N-morpholino)ethanesulfonic acid, 1 M Na+, and 20mM of EDT
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from hippocampus cells (wild type)
|
| Data processing |
GeneChip intensities were background-corrected, normalized and sumarized by RMA method, using the “Affy” package from Bioconductor.
|
| |
|
| Submission date |
Aug 27, 2009 |
| Last update date |
Apr 25, 2012 |
| Contact name |
Juan Carlos Oliveros |
| Organization name |
CNB, CSIC
|
| Street address |
Darwin 3
|
| City |
Cantoblanco |
| State/province |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE17844 |
Basal expression in daDREAM transgenic mouse hippocampus |
|
