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| Status |
Public on Jul 03, 2021 |
| Title |
Next Generation Sequencing (m6A-seq) Reveals RNA m6A Methylation Patterns During the Expansion of Tomato |
| Organism |
Solanum lycopersicum |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
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| Summary |
In this study, we show the potential link between m6A deposition and fruit expansion in tomato (Micro-tom). m6A level was increased during fruit expansion. Later, a parallel analysis of the m6A -seq and RNA-seq in expanding fruit were preformed to evaluate global correlation between m6A modification and fruit expansion. Our m6A -seq reveal that thousands of protein-coding genes contains m6A modification with a consensus motif and primarily enriched in the 3’untranslated regions. Gene ontology analysis indicate that the distribution of m6A in expanded fruit is largely associated with plant-specific processes relating to the chloroplast function. Moreover, we further uncover a positive correlation between m6A methylation and the mRNA abundance. Specifically, large number of fruit expansion-related genes, which involved in hormone and endoreplication, were m6A modified and actively expressed compared to non- m6A modified genes, suggesting a potential involvement of m6A modification in fruit expansion. Collectively, our result suggests a dynamic role of m6A modification involving in fruit expansion.
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| Overall design |
The MicroTom fruit samples were collected at 12, 20, 28 day after flowering. And the RNA extracted from fruit samples with two biological replicates was subjected to m6A and RNA sequencing.For data analysis,the early fruit sample at 12 day after folwering was used as reference sample to investigate the role of m6A in fruit expansion process.
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| Contributor(s) |
Kang H, Xu T |
| Citation(s) |
34730815 |
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| Submission date |
Jun 25, 2021 |
| Last update date |
Nov 24, 2021 |
| Contact name |
Tao Xu |
| E-mail(s) |
xutao_yr@126.com
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| Organization name |
Jiangsu Normal University
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| Street address |
Shanghai Road 101
|
| City |
Xuzhou |
| State/province |
Jiangsu |
| ZIP/Postal code |
221116 |
| Country |
China |
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| Platforms (1) |
| GPL27957 |
Illumina NovaSeq 6000 (Solanum lycopersicum) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA741478 |
| SRA |
SRP325630 |
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