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| Status |
Public on Jun 29, 2021 |
| Title |
DRG-CSC co-culture tissue engineered model, CSCs monoculture model and DRG monoculture model |
| Organisms |
Gallus gallus; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we constructed a tissue-engineered DRG-CSC (corneal stromal cells) co-cultured model (DS model), a CSC monoculture model (S model), and a DRG monoculture model (D model). Chicken DRGs were extracted from E7-E10 chicken embryo. Human CSCs were extracted in stromal lenticules from small incision lenticule extraction (SMILE). Transcriptional profiling of DRG in the DS model (DS-D), DRG in the D model(D-D), CSCs in the DS model (DS-S), CSCs in the S model (S-S) was analyzed. After 7 days of culture, RNA of each group was extracted.
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| |
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| Overall design |
DS-D vs D-D, DS-S vs S-S. There are three biological replicates in each group.
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| |
|
| Contributor(s) |
Cui Z, Chen J |
| Citation(s) |
35562005 |
| |
| Submission date |
Jun 28, 2021 |
| Last update date |
May 18, 2022 |
| Contact name |
Zekai Cui |
| E-mail(s) |
cuizk7@gmail.com
|
| Organization name |
Aier Eye Institute
|
| Street address |
18th floor, the New Century Building, #198, Furong Middle Road
|
| City |
Changsha |
| State/province |
Hunan |
| ZIP/Postal code |
410015 |
| Country |
China |
| |
|
| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL26853 |
Illumina NovaSeq 6000 (Gallus gallus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA741931 |
| SRA |
SRP325838 |
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