 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 23, 2022 |
| Title |
Cell-wall damage activates DOF transcription factors to promote wound healing and tissue regeneration in Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Wound healing is a fundamental property of plants and animals that requires recognition of cellular damage to initiate regeneration. In plants, wounding activates a defense response via the production of jasmonic acid and a regeneration response via the hormone auxin and several ethylene response factor (ERF) and NAC domain-containing protein (ANAC) transcription factors. To better understand how plants recognize damage and initiate healing, we searched for factors upregulated during the horticulturally relevant process of plant grafting and found four related DNA binding with one finger (DOF) transcription factors, HIGH CAMBIAL ACTIVITY2 (HCA2), TARGET OF MONOPTEROS6 (TMO6), DOF2.1, and DOF6, whose expression rapidly activated at the Arabidopsis graft junction. Grafting or wounding a quadruple hca2, tmo6, dof2.1, dof6 mutant inhibited vascular and cell-wall-related gene expression. Furthermore, the quadruple dof mutant reduced callus formation, tissue attachment, vascular regeneration, and pectin methylesterification in response to wounding. We also found that activation of DOF gene expression after wounding required auxin, but hormone treatment alone was insufficient for their induction. However, modifying cell walls by enzymatic digestion of cellulose or pectin greatly enhanced TMO6 and HCA2 expression, whereas genetic modifications to the pectin or cellulose matrix using the PECTIN METHYLESTERASE INHIBITOR5 overexpression line or korrigan1 mutant altered TMO6 and HCA2 expression. Changes to the cellulose or pectin matrix were also sufficient to activate the wound-associated ERF115 and ANAC096 transcription factors, suggesting that cell-wall damage represents a common mechanism for wound perception and the promotion of tissue regeneration.
|
| |
|
| Overall design |
The grafting transcriptomic dataset is consists of 36 samples, including col-0, dof quadruple, erf114,115 and anac071,096. Each genotype contains intact, scion and rootstock tissues, whereas each tissue of genotype is represented by three biological replicates.
|
| |
|
| Contributor(s) |
Zhang A, Melnyk CW |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 01, 2021 |
| Last update date |
Mar 23, 2022 |
| Contact name |
Ai Zhang |
| E-mail(s) |
ai.zhang@slu.se
|
| Organization name |
SLU
|
| Department |
Plant Biology
|
| Lab |
Charles Melnyk
|
| Street address |
Biocenter, Almas allé 5
|
| City |
Uppsala |
| State/province |
Uppsala |
| ZIP/Postal code |
75007 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
|
| Samples (36)
|
| GSM5413178 |
Scion Col-0 rep1 |
| GSM5413179 |
Scion Col-0 rep2 |
| GSM5413180 |
Scion Col-0 rep3 |
| GSM5413181 |
Rootstock Col-0 rep1 |
| GSM5413182 |
Rootstock Col-0 rep2 |
| GSM5413183 |
Rootstock Col-0 rep3 |
| GSM5413184 |
Intact dof quadruple rep1 |
| GSM5413185 |
Intact dof quadruple rep2 |
| GSM5413186 |
Intact dof quadruple rep3 |
| GSM5413187 |
Scion dof quadruple rep1 |
| GSM5413188 |
Scion dof quadruple rep2 |
| GSM5413189 |
Scion dof quadruple rep3 |
| GSM5413190 |
Rootstock dof quadruple rep1 |
| GSM5413191 |
Rootstock dof quadruple rep2 |
| GSM5413192 |
Rootstock dof quadruple rep3 |
| GSM5413193 |
Intact erf114,115 rep1 |
| GSM5413194 |
Intact erf114,115 rep2 |
| GSM5413195 |
Intact erf114,115 rep3 |
| GSM5413196 |
Scion erf114,115 rep1 |
| GSM5413197 |
Scion erf114,115 rep2 |
| GSM5413198 |
Scion erf114,115 rep3 |
| GSM5413199 |
Rootstock erf114,115 rep1 |
| GSM5413200 |
Rootstock erf114,115 rep2 |
| GSM5413201 |
Rootstock erf114,115 rep3 |
| GSM5413202 |
Intact anac071,096 rep1 |
| GSM5413203 |
Intact anac071,096 rep2 |
| GSM5413204 |
Intact anac071,096 rep3 |
| GSM5413205 |
Scion anac071,096 rep1 |
| GSM5413206 |
Scion anac071,096 rep2 |
| GSM5413207 |
Scion anac071,096 rep3 |
| GSM5413208 |
Rootstock anac071,096 rep1 |
| GSM5413209 |
Rootstock anac071,096 rep2 |
| GSM5413210 |
Rootstock anac071,096 rep3 |
|
| Relations |
| BioProject |
PRJNA743058 |
| SRA |
SRP326522 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE179283_Raw_Count_allsamples.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...