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Status |
Public on Jun 08, 2022 |
Title |
A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13 [Genome wide screen] |
Organism |
Escherichia coli str. K-12 substr. MG1655 |
Experiment type |
Other
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Summary |
The study investigates how varying target expression levels influences self-targeting and defense using Cas13a. We see how immunity is activated only over a target transcription threshold, which is variable between targets. A gRNA library targeting transcripts from the whole coding genome of E. coli MG1655 confirms the importance of the transcription threshold in determining immunity and shows that most of the cellular transcripts do not induce cytotoxicity.
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Overall design |
Comparison of enriched spacers between cells with and without Cas13a nuclease. Two biological replicates for each condition.
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Contributor(s) |
Vialetto E, Yu Y, Collins SP, Wandera KG, Barquist L, Beisel CL |
Citation missing |
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Submission date |
Jul 12, 2021 |
Last update date |
Sep 07, 2022 |
Contact name |
Chase Beisel |
E-mail(s) |
rsyn.hiri@gmail.com
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Organization name |
HIRI
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Street address |
Josef Schneider Strasse , D15
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City |
Wuerzburg |
ZIP/Postal code |
97080 |
Country |
Germany |
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Platforms (1) |
GPL21117 |
Illumina NextSeq 500 (Escherichia coli str. K-12 substr. MG1655) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE179915 |
A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13 |
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Relations |
BioProject |
PRJNA745681 |
SRA |
SRP327938 |