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Series GSE180637 Query DataSets for GSE180637
Status Public on Mar 10, 2023
Title A single-cell transcriptome landscape of the non-human primate placenta across pregnancy.
Organism Macaca fascicularis
Experiment type Expression profiling by high throughput sequencing
Summary The human placenta, a transient endocrine organ, is vital for successful pregnancy and fetal health, which undergoes dynamic cellular and functional changes during gestation. However, systematic longitudinal analysis of in utero human placenta formation and development across the lifespan is not feasible due to ethical and technical limit. To circumvent those difficulties and reveal the dynamic placentation, we obtain transcriptional snapshots of 217404 single cells from cynomolgus monkey placenta, decidua and embryo tissues spanning ten consecutive but distinct developmental stages across pregnancy. We reveal the development-related changes in the compositions of placental trophoblasts, mesenchymal cells and endothelial cells in different pregnant stage. We pinpoint that the placenta mesenchymal cells are derived from a special extra-embryonic mesoderm. Particularly, we describe the relationship between the embryonic primitive hematopoiesis and the placental endothelial cells, Hofbauer cells and erythrocytes. Besides, we found the cell types and their transcriptome compositions in decidual tissues show insignificant changes during the gestation. Finally, cell-cell interactions between trophoblasts and decidual cells, and between different cell types on villi are performed and suggested the mechanisms in dynamic placentation. Together, the high-resolution atlas we constructed would serve as a valuable resource to extrapolate key events for studies on human placentation and its associated disorders.
Overall design single cells from cynomolgus monkey placenta, decidua and embryo tissues spanning ten consecutive but distinct developmental stages across pregnancy. RNA libraries were prepared for sequencing using 10x Single Cell 3' Library & Gel Bead Kit v3 protocol
Contributor(s) Wang H, Jiang X, Zhai J, Xiao Z, Wan H, Xu Y, Wu X, Zhang D
Citation(s) 37054708
Submission date Jul 22, 2021
Last update date Apr 14, 2023
Contact name Xiangxiang Jiang
Phone 18810332066
Organization name Institute of Zoology, Chinese Academy of Sciences
Lab State Key Laboratory of Stem Cell and Reproductive Biology
Street address 1 Beichen West Road, Chaoyang District
City Beijing
State/province Beijing
ZIP/Postal code 100101
Country China
Platforms (1)
GPL23096 HiSeq X Ten (Macaca fascicularis)
Samples (23)
GSM5466489 E20-V1
GSM5466490 E23-D1
GSM5466493 E23-V1
BioProject PRJNA749013
SRA SRP329459

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE180637_MF-filtered.tar.gz 2.6 Gb (ftp)(http) TAR
GSE180637_MF217404.tar.gz 2.0 Gb (ftp)(http) TAR
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Raw data are available in SRA
Processed data are available on Series record

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