|
| Status |
Public on Mar 10, 2023 |
| Title |
E35-V2 |
| Sample type |
SRA |
| |
|
| Source name |
placenta
|
| Organism |
Macaca fascicularis |
| Characteristics |
developmental stage: day 35
|
| Treatment protocol |
no treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single cells were added to the 10x Chip channels. Captured cells were lysed and the released RNA was barcoded through reverse transcription in individual GEMs.
RNA libraries were prepared for sequencing using 10x Single Cell 3' Library & Gel Bead Kit v2 protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
MF217404.tar.gz
|
| Data processing |
Raw fastq files were processed with Cell Ranger 3.1.0 with default mapping arguments.
Reads were mapped to the Macaca_fascicularis_5.0 genome
The cellranger aggr command was executed to adjust the sequencing depth of different samples to the same level.
Genome_build: Macaca_fascicularis_5.0
Supplementary_files_format_and_content: MF217404.tar.gz: raw counts and metadata
Supplementary_files_format_and_content: MF-filtered.tar: raw counts data filtered by cellranger
|
| |
|
| Submission date |
Jul 22, 2021 |
| Last update date |
Mar 10, 2023 |
| Contact name |
Xiangxiang Jiang |
| E-mail(s) |
jzhongqiang@126.com
|
| Phone |
18810332066
|
| Organization name |
Institute of Zoology, Chinese Academy of Sciences
|
| Lab |
State Key Laboratory of Stem Cell and Reproductive Biology
|
| Street address |
1 Beichen West Road, Chaoyang District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL23096 |
| Series (1) |
| GSE180637 |
A single-cell transcriptome landscape of the non-human primate placenta across pregnancy. |
|
| Relations |
| BioSample |
SAMN20349840 |
| SRA |
SRX11522644 |
