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Series GSE181178 Query DataSets for GSE181178
Status Public on Aug 23, 2021
Title A single-cell morpho-transcriptomic map of brassinosteroid action in the Arabidopsis root
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary The effects of brassinosteroid signaling on shoot and root development have been characterized in great detail but did not identify a simple consistent positive or negative impact on a basic cellular parameter. Here we combined digital 3D single-cell shape analysis and single-cell mRNA sequencing to characterize root meristems and mature root segments of brassinosteroid-blind mutants and wildtype. These data demonstrate that brassinosteroid signaling neither affects cell volume nor cell proliferation capacity. Instead, brassinosteroid signaling is essential for the precise orientation of cell division planes and the extent and timing of anisotropic cell expansion. Moreover, we found that the cell-aligning effects of brassinosteroid signaling can propagate to normalize the anatomy of both adjacent and distant brassinosteroid-blind cells through non-cell-autonomous functions, which are sufficient to restore growth vigor. Finally, single-cell transcriptome data discern directly brassinosteroid-responsive genes from genes that can react non-cell-autonomously and highlight arabinogalactans as sentinels of brassinosteroid-dependent anisotropic cell expansion.
Overall design Single-cell RNA-seq experiments were performed on protoplast cells extracted from the root-tips of 6 day old Arabidopsis thaliana seedlings.
Contributor(s) Graeff M, Rana S, Wendrich JR, Dorier J, Eekhout T, Fandino AC, Guex N, Bassel GW, De Rybel B, Hardtke CS
Citation(s) 34358681
Submission date Jul 30, 2021
Last update date Aug 24, 2021
Contact name Bert De Rybel
Organization name VIB
Department Center for Plant Systems Biology
Lab Vascular Development
Street address Technologiepark 71
City Ghent
ZIP/Postal code 9052
Country Belgium
Platforms (1)
GPL26208 Illumina NovaSeq 6000 (Arabidopsis thaliana)
Samples (2)
GSM5492069 bri TRIPLE
GSM5492070 bri T-RESCUE
BioProject PRJNA750934
SRA SRP330542

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Supplementary file Size Download File type/resource
GSE181178_RAW.tar 132.7 Mb (http)(custom) TAR (of H5)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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