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| Status |
Public on Aug 23, 2021 |
| Title |
A single-cell morpho-transcriptomic map of brassinosteroid action in the Arabidopsis root |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The effects of brassinosteroid signaling on shoot and root development have been characterized in great detail but did not identify a simple consistent positive or negative impact on a basic cellular parameter. Here we combined digital 3D single-cell shape analysis and single-cell mRNA sequencing to characterize root meristems and mature root segments of brassinosteroid-blind mutants and wildtype. These data demonstrate that brassinosteroid signaling neither affects cell volume nor cell proliferation capacity. Instead, brassinosteroid signaling is essential for the precise orientation of cell division planes and the extent and timing of anisotropic cell expansion. Moreover, we found that the cell-aligning effects of brassinosteroid signaling can propagate to normalize the anatomy of both adjacent and distant brassinosteroid-blind cells through non-cell-autonomous functions, which are sufficient to restore growth vigor. Finally, single-cell transcriptome data discern directly brassinosteroid-responsive genes from genes that can react non-cell-autonomously and highlight arabinogalactans as sentinels of brassinosteroid-dependent anisotropic cell expansion.
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| Overall design |
Single-cell RNA-seq experiments were performed on protoplast cells extracted from the root-tips of 6 day old Arabidopsis thaliana seedlings.
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| Contributor(s) |
Graeff M, Rana S, Wendrich JR, Dorier J, Eekhout T, Fandino AC, Guex N, Bassel GW, De Rybel B, Hardtke CS |
| Citation(s) |
34358681 |
| Submission date |
Jul 30, 2021 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Bert De Rybel |
| E-mail(s) |
beryb@psb.vib-ugent.be
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| Organization name |
VIB
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| Department |
Center for Plant Systems Biology
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| Lab |
Vascular Development
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| Street address |
Technologiepark 71
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| City |
Ghent |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA750934 |
| SRA |
SRP330542 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE181178_RAW.tar |
132.7 Mb |
(http)(custom) |
TAR (of H5) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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