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Status |
Public on Jul 28, 2022 |
Title |
Parathyroid Hormone Treatment Effect on Gene Expression in Primary Calvarial Osteoblasts |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
The effect of parathyroid hormone (PTH) treatment was tested on osteoblasts prepared from calvaria of C57BL/6J mice 7-10 days old. Osteoblasts were treated with either human parathyroid hormone (1-34), bovine (D-Trp12,Tyr34) parathyroid hormone (7-34) amide or vehicle control for 4 hr. Gene expression effects were evaluated by DNA microarray.
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Overall design |
Mouse osteoblasts were prepared independently for each biological replicate. Three biological replicates were used for each treatment. Osteoblasts were treated with either 100 nM human parathyroid hormone (1-34), 1 µM bovine (D-Trp12,Tyr34) parathyroid hormone (7-34) amide or vehicle for 4 hr. Total RNAs were collected and 100 ng analyzed for gene expression using Affymetrix Mouse Gene 2.0 ST GeneChips.
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Contributor(s) |
Luttrell LM, Barth JL |
Citation missing |
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Submission date |
Aug 01, 2021 |
Last update date |
Jul 30, 2022 |
Contact name |
Jeremy Barth |
E-mail(s) |
barthj@musc.edu
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Organization name |
MUSC
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Street address |
173 Ashley Ave, BSB 627
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City |
Charleston |
State/province |
SC |
ZIP/Postal code |
29425-5080 |
Country |
USA |
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Platforms (1) |
GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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Samples (9)
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Relations |
BioProject |
PRJNA751401 |