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Series GSE181284 Query DataSets for GSE181284
Status Public on Jul 28, 2022
Title Parathyroid Hormone Treatment Effect on Gene Expression in Primary Calvarial Osteoblasts
Organism Mus musculus
Experiment type Expression profiling by array
Summary The effect of parathyroid hormone (PTH) treatment was tested on osteoblasts prepared from calvaria of C57BL/6J mice 7-10 days old. Osteoblasts were treated with either human parathyroid hormone (1-34), bovine (D-Trp12,Tyr34) parathyroid hormone (7-34) amide or vehicle control for 4 hr. Gene expression effects were evaluated by DNA microarray.
 
Overall design Mouse osteoblasts were prepared independently for each biological replicate. Three biological replicates were used for each treatment. Osteoblasts were treated with either 100 nM human parathyroid hormone (1-34), 1 µM bovine (D-Trp12,Tyr34) parathyroid hormone (7-34) amide or vehicle for 4 hr. Total RNAs were collected and 100 ng analyzed for gene expression using Affymetrix Mouse Gene 2.0 ST GeneChips.
 
Contributor(s) Luttrell LM, Barth JL
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Submission date Aug 01, 2021
Last update date Jul 30, 2022
Contact name Jeremy Barth
E-mail(s) barthj@musc.edu
Organization name MUSC
Street address 173 Ashley Ave, BSB 627
City Charleston
State/province SC
ZIP/Postal code 29425-5080
Country USA
 
Platforms (1)
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (9)
GSM5494191 Osteoblast-Vehicle-1
GSM5494192 Osteoblast-hPTH-1
GSM5494193 Osteoblast-bPTH-1
Relations
BioProject PRJNA751401

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE181284_RAW.tar 68.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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