|
| Status |
Public on Aug 15, 2023 |
| Title |
Gene Editing of Anp32a Confers Resistance to Avian Influenza in Chicken |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Comparison of transcriptomic profiles of primordial germ cells and derived fibroblast cell lines from homozygous, CRISPR/Cas9 gene-edited, ANP32A knockout and mutant chickens
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| |
|
| Overall design |
We used CRISPR/Cas9 to perform precise sequence changes in ANP32A to generate genome-edited PGC cell lines, which were also differentiated into fibroblast-like cell lines, all of which were subjected to RNA-seq in their native state.
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| |
|
| Contributor(s) |
Skinner MA, Idoko-Akoh A, McGrew M |
| Citation(s) |
37816720 |
| |
| Submission date |
Aug 18, 2021 |
| Last update date |
Oct 25, 2023 |
| Contact name |
Michael A Skinner |
| E-mail(s) |
m.skinner@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Faculty of Medicine, Section of Virology
|
| Street address |
St Mary's Campus, Norfolk Place
|
| City |
London |
| ZIP/Postal code |
W2 1PG |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL16133 |
Illumina HiSeq 2000 (Gallus gallus) |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA756092 |
| SRA |
SRP333188 |
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