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| Status |
Public on Aug 30, 2021 |
| Title |
The tetratricopeptide repeat and J-domain containing proteins DJC31 and DJC62 are involved in abiotic stress response in Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Molecular chaperones play an important role during the response to different stresses. Since plants are sessile organisms, they need to be able to adapt quickly to different conditions. To do so, plants possess a complex chaperone machinery, composed of HSP70, HSP90, J-proteins and other factors. In this study we characterized DJC31 and DJC62, two J-proteins that additionally carry clamp type tetratricopeptide repeat (TPR) domains. Using cell fractionation and self-assembly GFP, we could show that both proteins are attached to the cytosolic side of the endoplasmic reticulum membrane. Moreover, an interaction with cytosolic HSP70 and HSP90 could be shown by bimolecular fluorescence complementation. Knockout of either DJC31 or DJC62 caused only a mild phenotype, which is overall comparable to wild type. However, the double mutant exhibited severe defects in growth and development, which affected almost all organs. Furthermore, it could be shown that the double mutant is more sensitive to osmotic stress and treatment with abscisic acid, but surprisingly exhibited enhanced tolerance to drought. Taken together, these findings indicate that DJC31 and DJC62 might be acting as important regulators of chaperone dependent signalling pathways involved in plant development and stress response.
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| Overall design |
Examination of differential gene expression in the Arabdidopsis thaliana djc mutants compared to wild type in three replicates
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| Contributor(s) |
Schwenkert S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 27, 2021 |
| Last update date |
Aug 30, 2021 |
| Contact name |
Serena Schwenkert |
| E-mail(s) |
serena.schwenkert@lmu.de
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| Phone |
004989218074760
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| Organization name |
LMU
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| Department |
Biology
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| Lab |
Botany
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| Street address |
Grosshadernerstr. 2-4
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| City |
Planegg-Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA758370 |
| SRA |
SRP334464 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE182929_djc_counts_with_annotations.xlsx |
7.7 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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