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| Status |
Public on Sep 17, 2021 |
| Title |
Transcriptomic analysis of STAT1/3 in the goat endometrium during embryo implantation |
| Organism |
Capra hircus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goals of this study are to compare the differentially expressed genes in IFNT-treated and untreated goat endometrial epithelial cells and screen the signaling pathways related to endometrial receptivity.
Methods: We performed RNA sequencing of goat endometrial epithelial cells (gEECs) with or without 20 ng/mL IFNT treatment. KEGG enrichment analysis was used to screen for enrichment pathways, and qRT-PCR, western blotting and immunohistochemistry were used for in vivo and in vitro validation.
Results: Differential comparison showed that there were 442 upregulated differentially expressed genes (DEGs) and 510 downregulated DEGs. Bioinformatic analyses revealed that DEGs were significantly enriched in immune-related functions or pathways. The results of qRT-PCR, western blotting and immunohistochemistry were consistent with those of RNA-seq.
Conclusion: These results highlight the role of IFNT in regulating endometrial receptivity in gEECs and uncover the temporal and spatial changes in the expression of STAT1/3 during embryo implantation in the goat endometrium.
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| Overall design |
The mRNA profile of goat endometrial epithelial cells with or without 20 ng/mL IFNT treatment were generated by RNA sequencing, in triplicate, using Illumina HiSeq 4000.
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| Contributor(s) |
Liu H, Wang C, Li Z, Shang C, Zhang X, Zhang R, Wang A, Jin Y, Lin P |
| Citation(s) |
34722712 |
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| Submission date |
Sep 14, 2021 |
| Last update date |
Nov 04, 2021 |
| Contact name |
Haokun Liu |
| E-mail(s) |
lhk1995lhk@126.com
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| Organization name |
Northwest A&F University
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| Street address |
Xinong Road 22
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| City |
Yangling |
| ZIP/Postal code |
712100 |
| Country |
China |
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| Platforms (1) |
| GPL22146 |
Illumina HiSeq 4000 (Capra hircus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA763197 |
| SRA |
SRP337048 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE184110_mRNA_FPKM.xls.gz |
1.3 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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