|
| Status |
Public on Sep 17, 2021 |
| Title |
IFNT rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Goat endometrial epithelial cells
|
| Organism |
Capra hircus |
| Characteristics |
cell type: Goat endometrial epithelial cells
treatment: 20 ng/mL of IFNT treatment for 6h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of gEECs was isolated using RNAiso Plus (Takara, Japan), and genomic DNA was removed using the Evo M-MLV RT Kit (Accurate Biology, China) according to the manufacturer’s protocols.
RNA libraries were prepared for sequencing using standard lllumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Raw reads were aligned to the goat genome using HISA T2 (v2.0.4) software.
Transcriptome assembly was performed using StringTie (v1.3.1) software in a reference-based approach.
Gene expression abundance was assessed by calculating the FPKMs of genes in each sample using Cufflinks (v2.1.1) software.
Genome_build: Capra hircus (goat); ID: 10731
Supplementary_files_format_and_content: .xls file include FPKM values for each Sample
|
| |
|
| Submission date |
Sep 14, 2021 |
| Last update date |
Sep 17, 2021 |
| Contact name |
Haokun Liu |
| E-mail(s) |
lhk1995lhk@126.com
|
| Organization name |
Northwest A&F University
|
| Street address |
Xinong Road 22
|
| City |
Yangling |
| ZIP/Postal code |
712100 |
| Country |
China |
| |
|
| Platform ID |
GPL22146 |
| Series (1) |
| GSE184110 |
Transcriptomic analysis of STAT1/3 in the goat endometrium during embryo implantation |
|
| Relations |
| BioSample |
SAMN21434796 |
| SRA |
SRX12187859 |
