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| Status |
Public on Oct 27, 2021 |
| Title |
Divergent leukemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18 |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by array
Genome variation profiling by SNP array
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| Summary |
Hematopoietic malignancies are frequently characterized by karyotypic abnormalities. The development of targeted drugs has been pioneered with compounds against gene products of fusion genes caused by chromosomal translocations. While polysomies are equally frequent as translocations, for many of them we are lacking therapeutic approaches aimed at synthetic lethality. Here, we report two new cell lines, named MBU-7 and MBU-8, that differ in complete trisomies of chromosome 18, a partial trisomy of chromosome 7 and a tetrasomy of the p-arm of chromosome 8, but otherwise share the same mutational pattern and complex karyotype. Both cell lines are divergent clones of U-937 cells and have the morphology and immunoprofile of monocytic cells. The distinct karyotypic differences between MBU-7 and MBU-8 are associated with a difference in the specific response to nucleoside analogues. Taken together, we propose the MBU-7 and MBU-8 cell lines described here as suitable in vitro models for screening and testing vulnerabilities that are associated with the disease-relevant polysomies of chromosome 7, 8 and 18.
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| Overall design |
Two leukemia-derived cell lines were studied
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| Contributor(s) |
Maher M, Diesch J, Le Pannérer M, Cabezón M, Mallo M, Vergara S, López AM, Tudel AM, Solé F, Granada I, Sorigue M, Zamora L, Buschbeck M |
| Citation(s) |
34707142 |
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| Submission date |
Sep 24, 2021 |
| Last update date |
Nov 10, 2021 |
| Contact name |
Mar Mallo |
| Organization name |
Josep Carreras Leukaemia Research Institute
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| Street address |
Ctra de Can Ruti, s/n, camí de les escoles. Edifici IMPPC
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| City |
Badalona |
| ZIP/Postal code |
08916 |
| Country |
Spain |
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| Platforms (1) |
| GPL18637 |
[CytoScan750K_Array] Affymetrix CytoScan 750K Array |
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| Samples (2) |
| GSM5597156 |
Divergent leukemia sub-clones derived from U-937 cells (MBU-7) |
| GSM5597157 |
Divergent leukemia sub-clones derived from U-937 cells (MBU-8) |
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| Relations |
| BioProject |
PRJNA766076 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE184764_RAW.tar |
195.7 Mb |
(http)(custom) |
TAR (of CEL, CYCHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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