|
| Status |
Public on Oct 27, 2021 |
| Title |
Divergent leukemia sub-clones derived from U-937 cells (MBU-7) |
| Sample type |
genomic |
| |
|
| Source name |
Cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U-937 derived cell line MBU-7
disease state: leukemia
|
| Treatment protocol |
None
|
| Growth protocol |
All cell lines were cultured in RPMI 1640 (ThermoFisher Scientific) supplemented with 10%, 1% Pen/Strep, and 1% L-glutamine at 37ºC in 5% CO2. All cell lines were routinely analyzed for the presence of mycoplasma
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from collected cells using PureLink Genomic DNA Mini Kit (ThermoFisher Scientific)
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (ThermoFisher)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using ThermoFisher fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
Hybridized to CytoScan 750K
|
| Data processing |
The array image was acquired using ThermoFihser GeneChip® Operating Software. Copy number and SNP study was performed with the Chromosome Analysis Suite (ThermoFisher, versión 4.2).
Copy number and SNP data (CYCHP file)
|
| |
|
| Submission date |
Sep 24, 2021 |
| Last update date |
Oct 27, 2021 |
| Contact name |
Mar Mallo |
| Organization name |
Josep Carreras Leukaemia Research Institute
|
| Street address |
Ctra de Can Ruti, s/n, camí de les escoles. Edifici IMPPC
|
| City |
Badalona |
| ZIP/Postal code |
08916 |
| Country |
Spain |
| |
|
| Platform ID |
GPL18637 |
| Series (1) |
| GSE184764 |
Divergent leukemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18 |
|
