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Series GSE185666 Query DataSets for GSE185666
Status Public on Feb 01, 2022
Title Dual Chromatin Repressors Regulate Hippocampal Development
Organisms Mus musculus; synthetic construct
Experiment type Expression profiling by high throughput sequencing
Other
Summary The cell fate transition from radial glial-like (RGL) cells to neurons and astrocytes is crucial for development and pathological conditions. Two chromatin repressors- the enhancer of zeste homolog 2 and suppressor of variegation 4-20 homolog, are highly associated with RGL cells in the hippocampus, implicating hippocampal cell fate commitment underlying these epigenetic regulations. Using a double knock-out mouse model, we demonstrated that loss of both chromatin repressors in the RGL population leads to deficits in hippocampal development. At the molecular level, single nuclei RNA-Seq revealed differential gene expression and provided mechanistic insight that dual chromatin repressors are critical for the maintenance of cycling cells in the dentate gyrus as well as the balance of cell trajectories between neuronal and astroglial lineages.
 
Overall design Linked flow cytometry and single-nuclei RNAseq datasets from wild-type and double knock-out of Ezh2 and Suv4-20h mice brains
 
Contributor(s) Chang K, Rhodes CT, Abate AR, Lin CA
Citation(s) 34890048
Submission date Oct 11, 2021
Last update date May 06, 2022
Contact name Kai-Chun Chang
E-mail(s) kai-chun.chang@ucsf.edu
Organization name University of California, San Francisco
Department Bioengineering
Lab Abate
Street address 1700 4th St.
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platforms (2)
GPL17769 Illumina MiSeq (synthetic construct)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM5621394 cDNA fraction from wild-type and double knock-out nuclei
GSM5621395 nanowell index fraction
Relations
BioProject PRJNA770348
SRA SRP340849

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE185666_RAW.tar 2.9 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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