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| Status |
Public on May 10, 2023 |
| Title |
VAL1/2 recruit PKL to target sites in arabidopsis genome |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
PICKLE (PKL), a Chromodomain Helicase DNA binding domain type 3-type (CHD3) chromatin remodeler, noted for an embryonic structure called pickle root in primary root tip in pkl mutant, has been studied for decades. we obtained a comprehensive genome occupancy of PKL by Chromatin immunoprecipitation-sequencing (ChIP-seq), and found PKL co-occupy with the major repressors of seed maturation program, VIVIPAROUS1/ABI3-LIKE1/2 (VAL1/2) in genome. Furthermore, PKL physically interacts with VAL1/2 in vivo and phenotype and transcriptome data indicated that PKL and VAL1/2 function in a common pathway. Moreover, ChIP-seq and ChIP-qPCR results showed that VAL1/2 are necessary for the recruitment of PKL to co-target genes
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| Overall design |
Examination of PKL occupancy in 14-day-old WT and val1 val2 seedlings.
Examination of GFP occupancy in 14-day-old WT
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| |
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| Contributor(s) |
Liang Z, Li C |
| Citation missing |
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| Submission date |
Oct 19, 2021 |
| Last update date |
May 13, 2023 |
| Contact name |
Liang Zhenwei |
| E-mail(s) |
liangzhenw@sina.com
|
| Organization name |
Sun Yat-sen University
|
| Department |
School of Life Science
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| Street address |
Xingang West Road
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510075 |
| Country |
China |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA772752 |
| SRA |
SRP342157 |
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