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| Status |
Public on Apr 15, 2022 |
| Title |
Transcriptomic response in the heart and kidney to different types of antihypertensive drug administration [kidney] |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
To unravel antihypertensive drug-induced gene expression changes that are potentially related to the amelioration of end-organ damages, we performed in vivo phenotypic evaluation and transcriptomic analysis on the heart and the kidney, with administration of antihypertensive drugs to two inbred strains of (i.e., hypertensive and normotensive) rats. We chose to use six antihypertensive classes: enalapril (angiotensin converting enzyme inhibitor), candesartan (angiotensin receptor blocker), hydrochlorothiazide (diuretics), amlodipine (calcium-channel blocker), carvedilol (vasodilating beta-blocker) and hydralazine. In the tested rat strains, four of six drugs, including two renin-angiotensin system (RAS) inhibitors, were effective for BP lowering, whereas the remaining two drugs were not. Besides BP lowering, there appeared to be some inter-drug heterogeneity in phenotypic changes, such as suppressed body weight (Bw) gain and Bw-adjusted heart weight reduction. For the transcriptomic response, a considerable number of genes showed prominent mRNA expression changes either in a BP-dependent or BP-independent manner with substantial diversity between the target organs. Noticeable changes of mRNA expression were induced particularly by RAS blockade, e.g., for genes in the natriuretic peptide system (Nppb and Corin) in the heart and for those in the RAS/ kallikrein-kinin system (Ren and rat Klk1 paralogs) and those related to calcium ion binding (Calb1 and Slc8a1) in the kidney. This submission includes the kidney data.
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| Overall design |
40 samples in total which consist of 2 biological replicates (or rats) per each of the 20 conditions. This dataset is also available in ArrayExpress (E-MTAB-10672).
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| Contributor(s) |
Takeuchi F, Kato N |
| Citation(s) |
34879704 |
| |
| Submission date |
Nov 06, 2021 |
| Last update date |
Jul 17, 2022 |
| Contact name |
Fumihiko Takeuchi |
| E-mail(s) |
fumihiko@takeuchi.name
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| Organization name |
National Center for Global Health and Medicine
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| Department |
Research Institute
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| Street address |
1-21-1 Toyama
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| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
162-8655 |
| Country |
Japan |
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| Platforms (1) |
| GPL2896 |
GE Healthcare/Amersham Biosciences CodeLink™ Rat Whole Genome Bioarray |
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| Samples (40)
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| This SubSeries is part of SuperSeries: |
| GSE188350 |
Transcriptomic response in the heart and kidney to different types of antihypertensive drug administration |
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| Relations |
| BioProject |
PRJNA778414 |
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