 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 31, 2022 |
| Title |
Phytochrome B regulates reactive oxygen signaling during stress in plants. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Plants are essential for life on Earth converting light into chemical energy in the form of sugars. To adjust for changes in light intensity and quality, and to become as efficient as possible in harnessing light, plants evolved multiple light receptors, signaling, and acclimation mechanisms. In addition to altering plant metabolism, development and growth, light cues sensed by some photoreceptors, such as phytochromes, are also impacting many plant responses to biotic and abiotic stresses. Central for plant responses to different stresses are reactive oxygen species (ROS) that function as key signaling molecules. Recent studies demonstrated that respiratory burst oxidase homolog (RBOH) proteins that reside at the plasma membrane and produce ROS at the apoplast play a key role in plant responses to different biotic and abiotic stresses. Here we reveal that phytochrome B (phyB) and RBOHs function as part of a key regulatory module that controls ROS production, transcript expression, and plant acclimation to excess light stress. We further show that phyB can regulate ROS production during stress even if it is restricted to the cytosol, and that phyB, RBOHD and RBOHF co-regulate thousands of transcripts in response to light stress. Surprisingly, we found that phyB is also required for ROS accumulation in response to heat, wounding, cold, and bacterial infection. Taken together, our findings reveal that phyB plays a canonical role in plant responses to biotic and abiotic stresses, regulating ROS production, and that phyB and RBOHs function in the same regulatory pathway.
|
| |
|
| Overall design |
mRNA from 4 week old WT, phyB, rbohD and rbohF plants non-treated, after 10 min of white ligh or 10 min of red light (600-700nm)
|
| |
|
| Contributor(s) |
Fichman Y, Sengupta S, Xiong H, Azad RK, Hibberd JM, Liscum E, Mittler R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 12, 2021 |
| Last update date |
May 31, 2022 |
| Contact name |
Soham Sengupta |
| Organization name |
St. Jude Children's Research Hospital
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
|
| Samples (36)
|
|
| Relations |
| BioProject |
PRJNA780007 |
| SRA |
SRP345854 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE188732_normalized_counts.txt.gz |
5.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...