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Series GSE189038 Query DataSets for GSE189038
Status Public on Nov 24, 2021
Title Distinguishing between recruitment and spread of silent chromatin structures in Saccharomyces cerevisiae [I]
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary The formation of heterochromatin at HML, HMR, and telomeres in Saccharomyces cerevisiae involves two main steps: Recruitment of Sir proteins to silencers and their spread throughout the silenced domain. For the following datasets, we created a fusion protein between the heterochromatin protein Sir3 and the non-site-specific bacterial adenine methyltransferase M.EcoGII, with or without a 3xV5 epitope at the C-terminus. We performed ChIP-seq experiments (immunoprecipitated Sir3-M.EcoGII-3xV5) and MeDIP-seq experiments (immunoprecipitated m6A methylated DNA). We also used a temperature-sensitive allele of SIR3 (sir3-8) fused to M.ECOGII to induce m6A methylation for MeDIP-seq.
Overall design 32 samples total (24 in initial submission, 8 more in resubmission). Input and IP data for each experiment are included.
Contributor(s) Brothers M, Rine J
Citation(s) 35073254
Submission date Nov 17, 2021
Last update date Feb 23, 2022
Contact name Molly Brothers
Organization name University of California, Berkeley
Department Molecular and Cell Biology
Lab Rine Lab (440 Barker Hall)
Street address UC Berkeley 440 Barker Hall
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
Platforms (1)
GPL22715 Illumina MiniSeq (Saccharomyces cerevisiae)
Samples (32)
GSM5693567 210122_Crabby_Sir3-MEcoGII-3xV5_input
GSM5693568 210122_Crabby_Sir3-MEcoGII-3xV5_ChIP
GSM5693569 200808_Chase_no-EcoGII_m6A_input
This SubSeries is part of SuperSeries:
GSE190137 Distinguishing between recruitment and spread of silent chromatin structures in Saccharomyces cerevisiae
BioProject PRJNA781240
SRA SRP346595

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Supplementary file Size Download File type/resource
GSE189038_RAW.tar 2.0 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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