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| Status |
Public on Nov 13, 2009 |
| Title |
The metabolic responce to iron deficiency in Saccharomyces cerevisiae |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
Iron is an essential cofactor for enzymes involved in numerous cellular processes. We analyzed the metabolomes and transcriptomes of yeast grown in iron-rich and iron-poor media to determine which biosynthetic processes are altered when iron availability falls.
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| Overall design |
Saccharomyces cerevisiae DBY7286 strain was grown from very low density to mid-log phase (A600 = 0.5, approximately 18 hrs.) in defined-iron SD minimal medium containing only the supplements necessary to meet auxotrophic requirements. Defined-iron SD minimal media were prepared with yeast nitrogen base lacking iron and copper, supplemented with 1 µM copper sulfate, 25 mM MES pH 6.1, 1 mM Ferrozine (Fluka), and the indicated concentrations of ferrous ammonium sulfate 10 µM (low iron) or 300 µM (high iron). All cells were grown at 30°C with shaking and four independent cultures were prepared for each growth condition
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| Contributor(s) |
Philpott CC, Shakoury-Elizeh M, Protchenko O |
| Citation(s) |
20231268 |
| |
| Submission date |
Nov 13, 2009 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Caroline Philpott |
| E-mail(s) |
carolinep@intra.niddk.nih.gov
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| Organization name |
National Institutes of Health
|
| Department |
NIDDK
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| Street address |
10 Center Dr, Bldg.10-Rm.9B-05
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA121085 |
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