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| Status |
Public on Apr 19, 2023 |
| Title |
Single-cell RNA-sequencing reveals an intratumor heterogeneity in pancreatic adenocarcinoma [CUT&RUN] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We examined the intratumor heterogeneity in S2-VP10 xenograft by single-cell RNA-sequencing. We found a hierarchical tumor progression originating from ROR1high cells with a partial EMT gene signature. We also found that ROR1high cells have the strong capacity as tumor-initiating cells to support tumorigenecity, chemoresistance, and metastasis. CUT&RUN and ATAC-sequencing revealed that ROR1 gene expression is regulated through YAP-BRD4 axis. Together, these data uncover a role for ROR1high cells in tumor progression, suggesting that ablating ROR1high tumor-initiating cells may be the alternative therapeutic strategy in pancreatic adenocarcinoma.
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| Overall design |
Examination of 3 diffierent histone modifications using CUT&RUN in S2-VP10 cells and sorted cells from S2-VP10 xenograft.
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| Contributor(s) |
Yamazaki M |
| Citation(s) |
37096681 |
| |
| Submission date |
Dec 18, 2021 |
| Last update date |
May 01, 2023 |
| Contact name |
Masaya Yamazaki |
| E-mail(s) |
myamazaki@kumamoto-u.ac.jp
|
| Organization name |
Kumamoto university
|
| Lab |
Medical Biochemistry
|
| Street address |
1-1-1, Honjyo, Chuo-ku
|
| City |
Kumamoto city |
| ZIP/Postal code |
860-8556 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE191204 |
Single-cell RNA-sequencing reveals an intratumor heterogeneity in pancreatic adenocarcinoma |
|
| Relations |
| BioProject |
PRJNA790389 |
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