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Status |
Public on Nov 01, 2011 |
Title |
Id1 maintains embryonic stem cell self-renewal by upregulation of Nanog and repression of Brachyury expression |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Understanding the mechanism by which embryonic stem (ES) cells self-renew is critical for the realization of their therapeutic potential. Previously it had been shown that in combination with LIF, Id proteins were sufficient to maintain mouse ES cells in a self-renewing state. Here we investigate the requirement for Id1 in maintaing ES cell self-renewal and blocking differentiation. We find that Id1-/- ES cells have a propensity to differentiate and a decreased capacity to self-renew. Chronic or acute loss of Id1 leads to a down-regulation of Nanog, a critical regulator of self-renewal. In addition, in the absence of Id1, ES cells express elevated levels of Brachyury, a marker of mesendoderm differentiation. We find that loss of both Nanog and Id1 is required for the up-regulation of Brachyury, and Id1 maintains Nanog expression by blocking the expression of Zeb1, a repressor of Nanog transcription. These results identify Id1 as an important factor in the maintenance of ES cell self-renewal and suggest a plausible mechanism for its control of lineage commitment.
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Overall design |
Wild type and Id1-/- ES cells were grown on gelatin under normal self-renewing conditions (in the presence of serum and LIF).
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Contributor(s) |
Romero-Lanman EE, Chin Y, Pavlovic S, Benezra R |
Citation missing |
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Submission date |
Nov 23, 2009 |
Last update date |
Jan 08, 2019 |
Contact name |
Jeffrey Zhao |
Organization name |
Memorial Sloan Kettering Cancer Center
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Department |
Genomics Core
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Street address |
1275 York Avenue
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City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
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Platforms (1) |
GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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Samples (2) |
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Relations |
BioProject |
PRJNA120693 |