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| Status |
Public on Dec 26, 2021 |
| Title |
Large scale transposon co-option in the Caenorhabditis germline regulatory network [RNA-Seq] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The movement of repetitive elements in the germline creates widespread genomic alterations and pressure for resolution. Here we show that the Caenorhabditis clade took advantage of two transposon expansions by integrating hundreds of elements into its germline transcriptional network. We find that about one-third of C. elegans germline-specific promoters have been co-opted from CERP2 and CELE2 MITE elements and are regulated by HIM-17, a THAP domain-containing transcription factor related to a transposase. An ancestral CERP2 expansion took place in the common Caenorhabditis ancestor, concurrently with mutations in HIM-17 fixed by positive selection, whereas CELE2 expanded only in C. elegans. Through comparative analyses in C. briggsae, we find conservation as well as species-specific CERP2 co-option. Our work reveals the emergence of a novel transcriptional network driven by TE co-option and its impact on regulatory evolution.
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| Overall design |
4 single-end RNA-seq libraries were generated from polyA-selected RNA from C. elegans strains (wt and him-17 mutants).
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| Contributor(s) |
Carelli FN, Dong Y, Cerrato C, Appert A, Ahringer J |
| Citation(s) |
36525485 |
| Submission date |
Dec 23, 2021 |
| Last update date |
Jan 11, 2023 |
| Contact name |
Francesco Nicola Carelli |
| E-mail(s) |
fr.carelli@gmail.com
|
| Organization name |
University of Cambridge
|
| Lab |
Julie Ahringer
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| Street address |
Henry Wellcome Building of Cancer and Developmental Biology, Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 1QN |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18730 |
Illumina HiSeq 1500 (Caenorhabditis elegans) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE192540 |
Large scale transposon co-option in the Caenorhabditis germline regulatory network |
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| Relations |
| BioProject |
PRJNA791898 |
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