|
| Status |
Public on Dec 26, 2021 |
| Title |
elegans-him17, rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Whole worm
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
tissue: Whole worm
strain: him-17(me24)
genotype: him-17 mutant
developmental stage: young adult
growth condition: 20°C
|
| Growth protocol |
For each of two replicates, approximately 100 wild-type and him-17(me24) (m+z-) young adults grown on agar plates at 20ºC from the starved L1 stage were hand-picked. him-17(me24) were derived from him-17(me24)/tmC12 [tmIs1194] mothers.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted using TriPure (Roche) and further purified using an RNeasy column (Qiagen).
poly A was isolated using the NEBNext Poly(A) mRNA Isolation kit and libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit (E7760S).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Data processing |
C. elegans RNA-seq reads were aligned to the WS275 C. elegans genome assembly using STAR (2.7.5a) with the TwoPass mode. Strand-specific wiggle output was converted to BigWig.
Genome_build: WS275
Supplementary_files_format_and_content: Forward and reverse-strand genome-wide read coverage in RPM (reads per million of mapped reads)
|
| |
|
| Submission date |
Dec 23, 2021 |
| Last update date |
Dec 26, 2021 |
| Contact name |
Francesco Nicola Carelli |
| E-mail(s) |
fr.carelli@gmail.com
|
| Organization name |
University of Cambridge
|
| Lab |
Julie Ahringer
|
| Street address |
Henry Wellcome Building of Cancer and Developmental Biology, Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 1QN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL18730 |
| Series (2) |
| GSE192538 |
Large scale transposon co-option in the Caenorhabditis germline regulatory network [RNA-Seq] |
| GSE192540 |
Large scale transposon co-option in the Caenorhabditis germline regulatory network |
|
| Relations |
| BioSample |
SAMN24366030 |
| SRA |
SRX13496428 |
