|
| Status |
Public on May 24, 2022 |
| Title |
Landscape of open chromatin regions in Rice upon exposure to abiotic stresses |
| Organism |
Oryza sativa |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The goal of the study is to illustrate how various abiotic stresses reshape the chromatin architecture of the Rice genome to fine tune gene expression.
|
| |
|
| Overall design |
Oryza sativa L. Japonica nipponbare was grown in a plant growth chamber (Conviron®) at 28°C under a photoperiod of 16 h/8 h. 14-day old seedlings were subjected to heat (42°C for 30 min and 3 h), cold (4°C for 12 h and 24 h), and salt (250 mM NaCl for 2 h and 6 h) stress. The 14-day old seedlings grown at 28°C were taken as the control sample. Open chromatin was extracted using Formaldehyde Assisted Isolation of Regulatory Elements (FAIRE-seq) from two biological replicates. RNA-seq was performed on two biological replicates
|
| |
|
| Contributor(s) |
Raxwal VK, Singh S, Agarwal M, Riha K |
| Citation(s) |
35676681 |
| |
| Submission date |
Dec 29, 2021 |
| Last update date |
Jun 23, 2022 |
| Contact name |
Vivek Kumar Raxwal |
| E-mail(s) |
vivek.raxwal@ceitec.muni.cz
|
| Organization name |
Central European Institute of Technology
|
| Department |
Plant Molecular Biology
|
| Street address |
building A26, Kamenice 753/5
|
| City |
Brno |
| ZIP/Postal code |
62500 |
| Country |
Czech Republic |
| |
|
| Platforms (1) |
| GPL13160 |
Illumina HiSeq 2000 (Oryza sativa) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA793014 |
Less...
More...