|
| Status |
Public on Jul 28, 2023 |
| Title |
Context-dependent perturbations in chromatin folding and the transcriptome by cohesin and related factors [Hi-C] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Cohesin plays vital roles in chromatin folding and gene expression regulation, cooperating with such factors as cohesin loaders, unloaders, and the insulation factor CTCF. Although models of regulation have been proposed (e.g., loop extrusion), how cohesin and related factors collectively or individually regulate the hierarchical chromatin structure and gene expression remains unclear. We have depleted cohesin and related factors and then conducted a comprehensive evaluation of the resulting 3D genome, transcriptome and epigenome data. We observed substantial variation in depletion effects among factors at topologically associating domain (TAD) boundaries and on interTAD interactions, which were related to epigenomic status.
|
| |
|
| Overall design |
We used the in situ Hi-C protocol as described in Rao et al. (Rao et al., Cell, 2014, 10.1016/j.cell.2014.11.021). In brief, ~3 × 10^6 RPE cells were crosslinked with 1% formaldehyde for 10 min at room temperature, followed by an additional 5 min with 200 mM glycine in phosphate-buffered saline (PBS). Fixed cells were permeabilized in Hi-C lysis buffer (10 mM Tris-HCl, pH 8.0; 10 mM NaCl; 0.2% Igepal CA630; 1× protease inhibitor cocktail [Sigma]) on ice. The cells were treated with 100 U of MboI (New England Biolabs) for chromatin digestion, and the ends of digested fragments were labeled with biotinylated nucleotides followed by ligation. After DNA reverse crosslinking and purification, ligated DNA was sheared to a size of 300–500 bp using a Covaris S2 focused-ultrasonicator (settings: Duty Cycle, 10%; Intensity, 4; Cycles per Burst, 200; Duration, 55 sec). The ligated junctions were then pulled down with Dynabeads MyOne Streptavidin T1 beads (Thermo Fisher Scientific). The pulled-down DNA was end-repaired, ligated to sequencing adaptors, amplified on beads and purified using Nextera Mate Pair Sample Preparation Kit (Illumina) and Agencourt AMPure XP (Beckman Coulter). DNA was then sequenced to generate paired-end 150-bp reads using the Illumina HiSeq-2500 or X Ten system.
|
| Web link |
https://www.nature.com/articles/s41467-023-41316-4
|
| |
|
| Contributor(s) |
Nakato R, Sakata T, Bando M, Shirahige K |
| Citation(s) |
37726281 |
| |
| Submission date |
Feb 03, 2022 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Ryuichiro Nakato |
| E-mail(s) |
rnakato@iqb.u-tokyo.ac.jp
|
| Phone |
+81-3-5841-1471
|
| Organization name |
The University of Tokyo
|
| Department |
Institute for Quantitative Biosciences
|
| Lab |
Laboratory of Computational Genomics
|
| Street address |
1-1-1 Yayoi
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-0032 |
| Country |
Japan |
| |
|
| Platforms (1) |
|
| Samples (31)
|
|
| This SubSeries is part of SuperSeries: |
| GSE196450 |
Context-dependent perturbations in chromatin folding and the transcriptome by cohesin and related factors |
|
| Relations |
| BioProject |
PRJNA803067 |
| SRA |
SRP358053 |
Less...
More...