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| Status |
Public on Dec 23, 2010 |
| Title |
Expression data from epithelial cells during the process of multistep pancreatic carcinogenesis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The host antitumor immunity changes drastically during carcinogenesis. Intraductal papillary-mucinous neoplasm (IPMN) of the pancreas is a precursor lesion of pancreatic cancer and progresses according to adenoma-carcinoma sequence. We found that the host antitumor immune reaction changes from an immune response to immune tolerance between intraductal papillary-mucinous adenoma (IPMA) and intraductal papillary-mucinous carcinoma (IPMC).
In order to determine molecules affecting intraepithelial DC infiltration in IPMNs during multistep carcinogenesis, we examined the gene-expression profiles of entire transcripts of neoplastic cells at different stages.
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| Overall design |
We collected normal and neoplastic epithelial cells from frozen tissue sections (normal main pancreatic duct, IPMA, IPMC, and invasive carcinoma originating in IPMN) by laser microdissection, extracted total RNA from them, and analyzed their gene expression profiles using Affymetrix microarrays.
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| Contributor(s) |
Hiraoka N, Itoh R, Ishikawa Y, Yamada T, Hirohashi S, Kanai Y |
| Citation(s) |
20955708 |
| |
| Submission date |
Dec 24, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Nobuyoshi Hiraoka |
| E-mail(s) |
nhiraoka@ncc.go.jp
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| Phone |
+81-3-3542-2511
|
| Organization name |
National Cancer Center Research Institute
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| Department |
Pathology Division
|
| Street address |
5-1-1 Tsukiji, Chuo-ku
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| City |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA122545 |
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