|
| Status |
Public on Dec 23, 2010 |
| Title |
normal main pancreatic duct 3 |
| Sample type |
RNA |
| |
|
| Source name |
microdissected epithelial cells from normal main pancreatic duct 3
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: pancreas
cell type: epithelial cells
disease status: normal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was obtained with Trizol extraction and purified with RNeasy Micro Kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30 ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 8000.
|
| Description |
Gene expression data from microdissected epithelial cells of normal main pancreatic duct
|
| Data processing |
The data were analyzed with GeneChip Operating Software version 1.4 (GCOS1.4) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
|
| |
|
| Submission date |
Dec 24, 2009 |
| Last update date |
Dec 23, 2010 |
| Contact name |
Nobuyoshi Hiraoka |
| E-mail(s) |
nhiraoka@ncc.go.jp
|
| Phone |
+81-3-3542-2511
|
| Organization name |
National Cancer Center Research Institute
|
| Department |
Pathology Division
|
| Street address |
5-1-1 Tsukiji, Chuo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE19650 |
Expression data from epithelial cells during the process of multistep pancreatic carcinogenesis |
|
